Age-dependency of alpha- and beta-adrenoceptors on thrombocytes and lymphocytes of asthmatic and nonasthmatic children
Identifieur interne : 003616 ( Main/Exploration ); précédent : 003615; suivant : 003617Age-dependency of alpha- and beta-adrenoceptors on thrombocytes and lymphocytes of asthmatic and nonasthmatic children
Auteurs : D. Reinhardt [Allemagne] ; T. Zehmisch [Allemagne] ; B. Becker [Allemagne] ; M. Nagel-Hiemke [Allemagne]Source :
- European Journal of Pediatrics [ 0340-6199 ] ; 1984-06-01.
English descriptors
- KwdEn :
- Teeft :
- Adenylate cyclase, Adrenergic, Adrenergic drugs, Adrenergic receptors, Agonist, Airway obstruction, Allergy clin, Ascorbic acid, Asthma, Asthmatic, Asthmatic child, Asthmatic children, Asthmatic children data, Asthmatic episodes, Asthmatic subjects, Beta adrenergic receptors, Binding assay, Binding data, Binding sites, Binding studies, Blood cells, Blood sample, Bronchial asthma, Catecholamine, Clin, Control infants, Different concentrations, Equal volume, Free ligand, General phenomenon, Human lymphocytes, Icyp, Individual values, Ipratropium bromide, Large amounts, Least squares, Ligand, Lymphocyte, Lymphocyte band, Lymphocyte subpopulations, Maturation process, Moderate asthma, Nonspecific binding, Number binding sites, Other tissues, Platelet, Present study, Receptor, Regression lines, Salbutamol, Saturation curve, Scatchard, Scatchard plot, Scatchard plots, Several authors, Single class, Small infants, Specific binding, Specific ligands, Statistical analysis, Thrombocyte.
Abstract
Abstract: Among the possible mechanisms which may cause wheezing or asthmatic episodes a genetically determined β-adrenoceptor blockade and a hyperresponsiveness of α-andrenoceptors has been postulated. Evidence to support this hypothesis stems from an increased bronchial sensitivity to β-blockers, a reduced formation of cyclic AMP in response to β-adrenergic stimulation and enhanced α-adrenergic responses in asthmatic subjects. The recent development of techniques for measuring the specific, high-affinity binding of radiolabeled α-and β-adrenergic antagonists made it possible to study α- and β-adrenoceptors in vitro. Based upon the assumption that a change in the number and/or affinity of adrenergic receptors might be a general phenomenon, we have performed α- and β-receptor binding studies on lymphocytes and platelets from wheezing infants and asthmatic children as well as of infants, children, and adults not suffering from these diseases. Using 125[I]-cyanopindolol (ICYP) and 3[H]-yohimbine (HYOH) as highly specific ligands for α- and β-adrenoceptors, the following results were obtained: (1) Lymphocytes and platelets from control subjects and asthamatics bound similar amounts of ICYP and HYOH and thus showed no differences either in the number or the affinity of α- and β-adrenoceptors. Lymphocytes and platelets of wheezing and nonwheezing infants also bound the same amounts of the radioligands. (2) In asthmatic children receiving 4×2 puffs salbutamol β-adrenoceptor were down-regulated and this may mimic β-adrenoceptor blockade. (3) When subjects were divided into four categories according to age (0–5, 5–10, 10–20 years, adults) the number of β-adrenoceptor binding sites showed an age-dependent increase. The number and affinity of α-adreneceptor binding sites on platelets was neither influenced by age nor disease. It is concluded that the α- and β-adrenoceptors of wheezing infants and asthmatic children at least on blood cells are normal. However the β-adrenoceptors show an age-dependent maturation process, which may account for an unresponsiveness to β-adrenoceptor agonists in wheezing infants.
Url:
DOI: 10.1007/BF00445589
Affiliations:
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Le document en format XML
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<front><div type="abstract" xml:lang="en">Abstract: Among the possible mechanisms which may cause wheezing or asthmatic episodes a genetically determined β-adrenoceptor blockade and a hyperresponsiveness of α-andrenoceptors has been postulated. Evidence to support this hypothesis stems from an increased bronchial sensitivity to β-blockers, a reduced formation of cyclic AMP in response to β-adrenergic stimulation and enhanced α-adrenergic responses in asthmatic subjects. The recent development of techniques for measuring the specific, high-affinity binding of radiolabeled α-and β-adrenergic antagonists made it possible to study α- and β-adrenoceptors in vitro. Based upon the assumption that a change in the number and/or affinity of adrenergic receptors might be a general phenomenon, we have performed α- and β-receptor binding studies on lymphocytes and platelets from wheezing infants and asthmatic children as well as of infants, children, and adults not suffering from these diseases. Using 125[I]-cyanopindolol (ICYP) and 3[H]-yohimbine (HYOH) as highly specific ligands for α- and β-adrenoceptors, the following results were obtained: (1) Lymphocytes and platelets from control subjects and asthamatics bound similar amounts of ICYP and HYOH and thus showed no differences either in the number or the affinity of α- and β-adrenoceptors. Lymphocytes and platelets of wheezing and nonwheezing infants also bound the same amounts of the radioligands. (2) In asthmatic children receiving 4×2 puffs salbutamol β-adrenoceptor were down-regulated and this may mimic β-adrenoceptor blockade. (3) When subjects were divided into four categories according to age (0–5, 5–10, 10–20 years, adults) the number of β-adrenoceptor binding sites showed an age-dependent increase. The number and affinity of α-adreneceptor binding sites on platelets was neither influenced by age nor disease. It is concluded that the α- and β-adrenoceptors of wheezing infants and asthmatic children at least on blood cells are normal. However the β-adrenoceptors show an age-dependent maturation process, which may account for an unresponsiveness to β-adrenoceptor agonists in wheezing infants.</div>
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